![]() Comparability studies (determine particle size distribution across different batches, lots, processes, perform stress testing of biologics)įor more information on these applications or the DLS analysis services by 2bind, take a look at our Application Database or contact our DLS specialists.ĭLS in general and with the Prometheus Panta in particular offers a great number of benefits:.Small molecule quality control (determine particle sizes after reconstituion, dilution, mixing, isothermally and across temperature gradients).Biologics development (characterize protein unfolding and aggregation, get self- and non-specific interactions, analyze protein size and homogeneity).Process development (determine and compare protein aggregation, characterize particle sizing and size distribution during process scale-up and optimization).Pre-formulation and formulation development (determine aggregation propensity, determine thermal stability, perform buffer and excipient screening, characterize particle sizing and solution homogeneity).Antibody engineering (determine aggregation propensity, get self- and non-specific interactions).Thus a broad image of comprehensive protein stability pathways can be obtained from a single experiment.ĭue to the robust detection method and quick analysis pace, Prometheus Panta is very versatile and can be used in a number of contexts: With the new Prometheus Panta device it is possible for the first time to measure colloidal (DLS) and conformational (nanoDSF/DLS) stability in one experiment with minimal sample volume (10 µl) and time (1.5 hours for 48 capillaries with nanoDSF and DLS data). This enables the rapid measurement of particles of different types and chemistry in solution and without expensive sample pre-treatment: Proteins, peptides, nucleic acids, viruses, micelles, lipids, liposomes, aggregations, emulsions can be measured.Ĭonsequently, DLS is a great tool for buffer and formulation screening as well as screening of small molecule compound libraries for influence on colloidal protein stability and aggregation tendency. Dynamic light scattering utilizes the elastic scattering of light (also known as Raleigh scattering) to determine the diffusion coefficients and hence the hydrodynamic radii of macromolecules. It does not store any personal data.DLS stands for dynamic light scattering and is the method of choice for particle sizing and optimization of colloidal sample stability. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. This cookie is used by our translation plugin "qTranslate-X" to offer our site in German and English and to save your language preferences. Spam protection: Stores dynamic variables from the browser The cookies is used to store the user consent for the cookies in the category "Necessary". This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Analytics". ![]() The cookies is used to store the user consent for the cookies in the category "Advertisement". Сookie to validate other cookies, so they can’t be spoofed. These cookies do not store any personal information. This category only includes cookies that ensures basic functionalities and security features of the website. Necessary cookies are absolutely essential for the website to function properly.
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